Скачать книгу

3.11A. I461/I537 has a linear relationship with the concentration of aluminum ions (Figure 3.11B). The lowest detection limit measured under the experimental conditions is 0.29 μM (7.8 ppb), and the fluorescence response is stable in the pH range of 3.0–10.0. DLS showed that the average diameter of aggregates decreased significantly before and after the interaction with aluminum ions, indicating that aluminum ions and probe 18 could form a water‐soluble complex, but there were still aggregates of probe 18 in the solution, thus showing that a white color fluorescence originated from the mixed color of the probe aggregate and the complex. The coordination ratio between probe 18 and the aluminum ion is 1 : 1 based on Job's plot; NMR titration indicates that both hydroxyl groups in the probe molecule participate in the coordination with the aluminum ion. Selectivity experiments with other metal ions show that a significant increase in I461/I537 was only found in the presence of Al3+ (Figure 3.11C). HeLa cells were selected for intracellular experiments, and the fluorescence signal was only observed in the yellow light channel. With the addition of aluminum ions, the yellow light signal was basically unchanged under the confocal microscope, and the fluorescence of the blue light channel gradually increased, showing that probe 18 can successfully perform ratio imaging of aluminum ion in cells (Figure 3.11D).

Image described by caption.

      Source: Reprinted from Ref. [27] (Copyright 2009 Elsevier B.V.).

       3.2.2 Biologically and Environmentally Related Molecular Detection and Imaging

Image described by caption.

      Source: Reprinted from Ref. [28] (Copyright 2010 John Wiley & Sons, Ltd.).

Image described by caption.

      Source: Reprinted from Ref. [26] (Copyright 2014 Elsevier B.V.).

Скачать книгу