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land, perennial grasslands can also be used for cultivation which increases biodiversity. Additionally, forest and agricultural residues used as biofuel feedstocks can reduce the negative impact on biodiversity. In algal biofuel, the large scale cultivation of algae could cause risk to coastal biodiversity due to attack of algae on coastal shallow ecosystems like mud flats, salt marshes, sea grass bed and coral reefs. Biodiversity loss can be identified as environmental concern therefore, preserving biodiversity is very important for sustainability of environment.

       5) Other environmental impacts

      Production and use of biodiesel may result emissions of particulate matter, SO₂, CO, NO_x, and various volatile organic carbons which results directly or indirectly in respiratory and other cardiovascular diseases [63]. Other environmental impacts depend on assumptions like type of vehicle in which biofuels are used, acidification and eutrophication of land and lakes. These impacts arise due to use of fertilizers and associated emissions of acid gases and nutrients to air and water.

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