LITMIR.BIZ

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(13)^b) TT (54.6)^b) (167)^b) (2.8)^b) (17)^b) AC (35.8)^b) (106)^b) (2.9)^b) (9)^b) CC (55.3)^b) (171)^b) (2.3)^b) (15)^b) 5′CAACTTGATATTAATA + Mismatch 3′GTTGAACTATAATTAT – 102.1 289 12.4 55.8 3′GTTGAGCTATAATTAT TG 92.6 266 10.1 49.4 3′GTTGAACTATAGTTAT TG 95.5 274 10.5 50.5 3′GTTGAACTCTAATTAT TC 98.4 286 9.7 47.3 3′GTTGAATTATAATTAT GT 91.3 264 9.4 47.1 3′GTTGAACCATAATTAT AC 90.9 265 8.7 44.6 3′GTTGAACAATAATTAT AA 92.0 267 9.26 46.2

a) Values are summarized ones in a reference [6].

b) Values in parenthesis are significantly less accurate estimated using flat lower baselines in their melting analyses.

Table 2.2 Free energy increments for tandem mismatches in RNA oligonucleotides in 1M NaCl^a), ^b).

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Mismatch	YZ ZY
5′ CGX YZX′ CG 3′		UG	GU	GA	AG	UU	GG	CA	CU	UC	CC	AC	AA
3′ GCX′ ZYX GC 5′		GU	UG	AG	GA	UU	GG	AC	UC	CU	CC	CA	AA
Closing base pair
X/X′	G/C	−4.9	−4.1	−2.6	−1.3	−0.5		1.0	1.1			0.9	1.5
	C/G	−4.2	−1.1	−0.7	−0.7	−0.4	0.8	1.1	1.4	1.4	1.7	2.0	1.3