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Secondary Metabolites of Medicinal Plants. Bharat Singh
Читать онлайн.Название Secondary Metabolites of Medicinal Plants
Год выпуска 0
isbn 9783527825592
Автор произведения Bharat Singh
Жанр Химия
Издательство John Wiley & Sons Limited
The biotransformation of betuligenol into an oxidized product betuloside was performed in the hairy roots of A. belladonna. The yield of biotransformed products increased twofold higher than the control root mass. On the 5th and 10th days of incubation, the highest biotransformation of betuligenol into raspberry ketone and betuligenol into betuloside was reported in hairy roots. The application of hairy roots for biotransformation of raspberry ketone and betuloside opens up new opportunities in the production of medicinally significant secondary metabolites (Srivastava et al. 2013).
The pharmaceutically important tropane alkaloids were isolated from the A. belladonna. The putrescine N-methyltransferase from N. tabacum and hyoscyamine 6β-hydroxylase from Hyoscyamus niger were overexpressed in transgenic A. belladonna for investigation of biosynthesis of tropane alkaloids in stem roots and fruits. The expression of putrescine N-methyltransferase and hyoscyamine 6β-hydroxylase was not reported in wild plant species but were found transgenic plants. The maximum conversion of hyoscyamine was observed in aerial parts, not in underground parts. In aerial parts, the hyoscyamine was fully transformed into scopolamine. The synthesis of hyoscyamine, anisodamine, and scopolamine was reported to have higher yield in underground parts than in aerial parts (Xia et al. 2016).
The effects of KCr(SO4)2 on the accumulation of tropane alkaloids as well as the expression of hyoscyamine 6β-hydroxylase gene was investigated in in vitro regenerated plantlets of A. belladonna. The chromium treatment to plantlets decreased the weights, lengths of the plantlets, and chlorophyll contents but enhanced the levels of proline contents. The chromium treatment also increased the concentration of hyoscyamine and scopolamine. The levels of scopolamine can be correlated with the expression levels of h6h gene with several concentrations of chromium (Jaber-Vazdekis et al. 2009; Vakili et al. 2012).
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