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on (Goodfellow and Johansson, 1984), and our present understanding about secular changes in the sulfur isotopic composition of oceanic sulfate during the past 130 million years is indeed based on a high‐resolution record of marine barite (Paytan et al., 2004).

      The most promising and widely utilized alternative, however, is carbonate‐associated sulfate (CAS). During carbonate precipitation, sulfate is incorporated into the crystal lattice in concentrations of tens to thousands of parts per million (Busenberg and Plummer, 1985; Staudt and Schoonen, 1995), and this sulfate archives the sulfur isotopic composition of the dissolved sulfate at the time of carbonate precipitation. As with true sulfate minerals, it is important to independently constrain the marine nature of carbonates used, such as marine biogenic carbonates. Similar to the modern ocean, limited variability in δ34SCAS can be expected for multiple time‐equivalent carbonate samples, as carbonate associated sulfate would reflect the homogeneous nature of the seawater sulfate sulfur isotopic composition at any given time in Earth history. However, recent studies have reported significant differences in δ34SCAS for different sedimentary carbonate components within an individual stratigraphic unit (Present et al., 2015), indicating that diagenesis can exert control on the δ34SCAS value of carbonates (Present et al., 2019). Consequently, a prerequisite for successfully applying δ34SCAS is a thorough evaluation of the diagenetic history of the carbonate (Fichtner et al., 2017), with a rigid analytical procedure (Wotte et al., 2012) being mandatory.

Schematic illustration of secular variations in delta 34S for carbonate-associated sulfate (CAS: blue circles) and evaporitic sulfate (orange circles).

      (Modified from Crockford et al., 2019.)

      Based on complimentary records of δ34Ssulfate and Δ33Ssulfate for Paleozoic and Mesozoic carbonate‐associated sulfate, and considering the temporal record of δ34Ssulfide through time, Wu et al. (2010, 2014) proposed significant changes in the operational mode of the global sulfur cycle through the Phanerozoic. Most notably, these authors highlighted a change in the magnitude of isotopic fractionation between sulfate and sulfide from lower values in the Paleozoic to higher values in the Cenozoic. Moreover, they argue for a change in the isotopic composition of the input function (δ34Sin) to the global sulfur cycle (i.e. the continental weathering signature), as previously discussed by Canfield (2013). Wu et al. (2014) attribute this to changes in the Earth surface sulfur pool, notably a rapid recycling of newly formed sulfate minerals. Discernible shorter‐term fluctuations (on the tens of million years scale) in the temporal records are interpreted as reflecting changes in the intensity of sulfide oxidation during cycling of sulfur and/or by rapid changes in sulfur influx to the oceans and its associated sulfur isotopes. Recently, Crockford et al. (2019) compiled and substantially extended the time series of δ34Ssulfate and Δ33Ssulfate for seawater sulfate (and δ18Osulfate and Δ17Osulfate) back in time into the late Archean, with new data mostly derived from gypsum or anhydrite. As in other previous studies, the observed large scale secular variations in the sulfur isotopic composition of seawater sulfate are attributed to temporal changes in burial/weathering of sedimentary sulfide. Moreover, these time series substantiate earlier suggestions (Melezhik et al., 2005) that the operational mode of the sulfur cycle as we know it today and, in particular, the continental weathering and riverine delivery of sulfate to the ocean only commenced in the early Proterozoic, postdating the first significant rise in atmospheric oxygen.

      Two additional aspects related to the study of carbonate‐associated sulfate are noteworthy as they likely reflect on future research. An increasing number of studies have focused on critical time boundaries such as the Permian–Triassic transition (Schobben et al., 2015, 2017), and careful work has resulted in high‐resolution profiles across prominent rock successions allowing for a renewed view on the causes and consequences of short‐term perturbations of global sulfur cycling. The second aspect pertains to an analytical improvement, notably the measurement of sulfur isotopes at very low sulfate concentrations (nanomole level) using multi‐collector induced coupled plasma mass‐spectrometry (MC‐ICP‐MS) (Craddock et al., 2008; Paris et al., 2013). Applications of the latter will allow for an even higher temporal resolution for rock successions across critical time boundaries than presently available.

      Being a key element of life, sulfur is taken up by plants and microorganisms via assimilatory reduction of inorganic sulfate with little sulfur isotope fractionation associated. In contrast, dissimilatory sulfate reduction is an energy‐yielding process associated with the release of hydrogen sulfide. Kaplan and Rittenberg (1964) in their seminal paper on isotopic fractionation associated with dissimilatory sulfate reduction established the foundation for the application of sulfur isotopes as recorder of microbial activities in the geological past. Hartmann and Nielsen (1969) were the first to apply this new understanding that dissimilatory sulfate reduction is generally associated with a substantial isotopic discrimination against the heavy 34S isotope in their study of marine sediments. Subsequently, a negative δ34S value measured for a sedimentary pyrite or at least a sizeable apparent isotopic fractionation between the parental sulfate (preserved in the rock record as evaporite) and resulting sedimentary (iron) sulfide, as observed in natural marine settings as well as in the early laboratory experiments, were considered as evidence for the biogenicity of a sedimentary sulfide: it provided the basis for tracing microbial sulfur cycling through time.

      Since the 1970s, numerous studies explored the antiquity of microbial sulfur cycling by studying sedimentary rocks as far back as 3.8 billion years ago (Monster et al., 1979). Time series of δ34S values for pyrite in sedimentary rocks were presented, among others, by Schidlowski et al. (1983), Hayes et al. (1992), Strauss (1999), and Canfield (2001a). Reviewing sulfur isotope research targeting sedimentary sulfides with the objective of identifying microbial sulfur cycling and trace it through time two milestone discoveries in the past 20 years by Sim et al. (2011) and Pellerin et al. (2019) are most notable.

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