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Erosion Superficial epithelial loss, not into dermis Erosive lichen planus Vesicle Fluid‐filled lesion up to 5 mm in diameter Herpes simplex Bulla Fluid‐filled lesion >5 mm Bullous pemphigoid Pustule Pus‐filled lesion Folliculitis Fissure Linear break in epithelium which can involve dermis if deep Mechanical hymenal fissure, psoriasis, lichen sclerosus, Crohn’s disease Comedone Keratin plug open to surface Hidradenitis suppurativa

      1 1 Lynch, P.J., Moyal‐Barracco, M., Scurry, J. and Stockdale, C.2011 ISSVD Terminology and classification of vulvar dermatological disorders: An approach to clinical diagnosis. J Low Genit Tract Dis. 2012 Oct; 16(4): 339–344.

      2 2 Lynch, P.J., Moyal‐Barracco, M., Bogliatto, F. et al. 2006 ISSVD classification of vulvar dermatoses: Pathologic subsets and their clinical correlates. J Reprod Med. 2007 Jan; 52(1): 3–9.

      3 3 Bohl, T.J. Vulvar ulcers and erosions: A clinical approach. Clin Obstet Gynecol. 2015; 58: 492–502.

       Fiona M. Lewis

      CHAPTER MENU

        Biopsy Reasons for taking a biopsy Site of biopsy Pre-biopsy Types of biopsy Punch biopsy Incisional biopsy Elliptical biopsy Excisional biopsy Shave biopsy Local anaesthesia Technique Post-biopsy instructions Samples Documentation What do you need to tell the pathologist?

        Cytology

        Microbiological investigation Swabs Scrapings Serology Wet mount microscopy

        Wood’s lamp examination

        Patch testing Resources

        Prick testing

        Blood tests

        Imaging

        Dermoscopy

        Vulvoscopy

        References

      With some clinical presentations, the diagnosis can be made on the basis of the history and examination alone. However, investigations may be necessary to confirm the clinical diagnosis and to gain further information in order to formulate an appropriate management plan. These investigations need to be tailored to the clinical features, as performing extensive investigations without a clinical differential diagnosis is never helpful.

      With any investigation, there must be good communication with the laboratory and the appropriate specialist, particularly with the histopathologist as clinicopathological correlation is crucial. If infection is suspected, special tests are often required, which may need specific collection techniques and transport media. This will require discussion with microbiology specialists and the local laboratory before taking specimens.

      Reasons for taking a biopsy

      A vulval biopsy is performed to confirm the clinical diagnosis, to help when there are a number of clinical differential diagnoses, or where the clinical features are atypical. A biopsy must always be done in the context of clinical diagnosis, and if sent in isolation without this, it is very easy to draw the wrong conclusion. The majority of biopsies are done for diagnostic reasons, but excisional biopsies can also be therapeutic.

      Most vulval biopsies are easily performed under local anaesthesia in the outpatient setting, but it is best to avoid biopsies of the clitoris, urethra, and anal margin. These patients should be referred to the relevant specialist – gynaecologist, urologist or colorectal surgeon – to consider biopsy under general anaesthetic.

      A clear drug history specifically asking about anticoagulants must be taken before the biopsy. Some patients may take low‐dose aspirin that is not prescribed, so this needs to be checked. Genetic clotting problems and allergies, especially to local anaesthetic, must be detailed.

      The procedure is

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